Words similar to bis
Example sentences for: bis
How can you use “bis” in a sentence? Here are some example sentences to help you improve your vocabulary:
MgSO 4 , (ethylene-bis [oxyethylenenitrilo])tetraacetic acid (EGTA), GTP (type II-S), glycerol, 2-( N -morpholino)-ethane sulfonic acid (MES), Nonidet P 40, piperazine- N,N '-bis(2-ethanesulfonic acid) (PIPES) and Triton X-100 were purchased from Sigma Chemical Company.
BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).
Prehybridization was performed in a solution containing 60% formamide, 0.1 M piperazine- N,N' -bis(2-ethanesulfonic acid) pH 6.4, 0.4 M NaCl, and 0.2 mg/ml of E. coli tRNA (Sigma, St. Louis, MO) for 2 h at 55°C with gentle shaking.
To disrupt the actin filaments of the cytoskeleton, the cell monolayer was treated with 25 nM cytochalasin D for 20 min at 37°C; TGFβ 1 was then applied in the presence of 25 nM cytochalasin D. For chelating intracellular calcium, the cells were preincubated with 5 μM of 1,2-bis(2-aminophenoxy)ethane- N , N , N ', N '-tetraacetic acid, acetoxymethyl ester (BAPTA-AM) for 15 min.
Derivatization was completed by formation of the trimethylsilyl derivatives by treatment with N,O-bis (trimethylsilyl) trifluoroacetamide and pyridine.
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