Words similar to bis
Example sentences for: bis
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Initial results showed that CAR is an apparently constitutive transactivator able to activate gene expression in the absence of exogenously added ligands [ 8 9 ] . The first CAR ligands identified, androstanol and androstenol, are inverse agonists that block this constitutive transactivation [ 10 ] . More recently, it has been found that CAR mediates the characteristic response of a subset of drug metabolizing enzymes to a variety of structurally unrelated foreign compounds [ 11 ] . These xenobiotic activators are referred to as "phenobarbital (PB) - like," based on the prototypic effects of the anti-epileptic agent on drug metabolism [ 12 13 14 ] . The most potent activator of murine CAR is the high affinity agonist ligand TCPOBOP (1,4-bis [2-(3,5-dichloropyridyloxy)]benzene) [ 15 16 ] .
Derivatization was completed by formation of the trimethylsilyl derivatives by treatment with N,O-bis (trimethylsilyl) trifluoroacetamide and pyridine.
To disrupt the actin filaments of the cytoskeleton, the cell monolayer was treated with 25 nM cytochalasin D for 20 min at 37°C; TGFβ 1 was then applied in the presence of 25 nM cytochalasin D. For chelating intracellular calcium, the cells were preincubated with 5 μM of 1,2-bis(2-aminophenoxy)ethane- N , N , N ', N '-tetraacetic acid, acetoxymethyl ester (BAPTA-AM) for 15 min.
MgSO 4 , (ethylene-bis [oxyethylenenitrilo])tetraacetic acid (EGTA), GTP (type II-S), glycerol, 2-( N -morpholino)-ethane sulfonic acid (MES), Nonidet P 40, piperazine- N,N '-bis(2-ethanesulfonic acid) (PIPES) and Triton X-100 were purchased from Sigma Chemical Company.
Prehybridization was performed in a solution containing 60% formamide, 0.1 M piperazine- N,N' -bis(2-ethanesulfonic acid) pH 6.4, 0.4 M NaCl, and 0.2 mg/ml of E. coli tRNA (Sigma, St. Louis, MO) for 2 h at 55°C with gentle shaking.
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