Words similar to bis
Example sentences for: bis
How can you use “bis” in a sentence? Here are some example sentences to help you improve your vocabulary:
To disrupt the actin filaments of the cytoskeleton, the cell monolayer was treated with 25 nM cytochalasin D for 20 min at 37°C; TGFβ 1 was then applied in the presence of 25 nM cytochalasin D. For chelating intracellular calcium, the cells were preincubated with 5 μM of 1,2-bis(2-aminophenoxy)ethane- N , N , N ', N '-tetraacetic acid, acetoxymethyl ester (BAPTA-AM) for 15 min.
After evaporation of reagents, the residue was subjected to TLC plates, using the solvent system chloroform/ethanol (93:7, vol/vol) for PGF 2α and TXB 2 , and ethyl acetate/methanol (93:2, vol/vol) for 6-keto-PGF 1α and PGE 2 . Then PGF 2α was converted to trimethylsilyl ether derivative by treatment with N,O-bis (trimethylsilyl) trifluoroacetamide and dimethylformamide.
The supernatant was assayed for caspase activity in 20 mM Piperazine-N,N'-bis(2-ethanesulfonic acid) (pH 6.8), 100 mM NaCl, 1 mM EDTA, 1% CHAPS, and 10% sucrose with Z-IETD-AFC for caspase 8 [ 42 43 ] . The release of the fluorogenic-leaving group was detected by excitation at 400 nm and emission at 505 nm.
BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).
Stores such as Dorothée-Bis, Benetton, and the less expensive but lower quality Tati are found throughout the capital.
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