Words similar to bis
Example sentences for: bis
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Protein A, the protease inhibitors phenylmethyl-sulfonyl fluoride, leupeptin and pepstatin, and 2,3-bis [2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) were all from Sigma, St. Louis, MO.
Pyridine-2,6-bis(thiocarboxylic acid) (pdtc) is an iron-regulated metabolite produced by certain pseudomonads that is highly reactive towards metals by virtue of a pair of thiocarboxylate groups and ring nitrogen that combine to form a non-specific, high affinity, tri-dentate ligand (Figure 1) [ 1 2 ] . The formation constants for Co, Cu, and Fe complexes of pdtc were determined to be greater than 10 33 [ 3 ] . Uptake studies using 59Fe showed that addition of pdtc to the growth medium of two different pdtc-producing pseudomonads increased the efficiency of iron uptake by 20% [ 1 ] . The contribution of pdtc to iron uptake may be due to the fact that the copper complex of pdtc (Cu:pdtc) has the ability to reduce both soluble and mineral forms of ferric iron) [ 1 ] . Not surprisingly, in light of its high affinity for essential metals, pdtc also exhibits antimicrobial activity [ 4 ] .
The supernatant was assayed for caspase activity in 20 mM Piperazine-N,N'-bis(2-ethanesulfonic acid) (pH 6.8), 100 mM NaCl, 1 mM EDTA, 1% CHAPS, and 10% sucrose with Z-IETD-AFC for caspase 8 [ 42 43 ] . The release of the fluorogenic-leaving group was detected by excitation at 400 nm and emission at 505 nm.
Crystals were grown by the hanging drop method in which 2 μl of the 7.5 mg/ml dimer solution in 10 mM bis tris propane (pH 8.5) and 200 mM NaCl, were mixed with 2 μl of reservoir solution containing 5% PEG 4000 and 2.0 M ammonium sulfate.
4Bis caused by errors in these long time blood measurements.
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