Example sentences for: biosystems

How can you use “biosystems” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Matching primers and fluorescence probes were designed for each of the genes using the Primer Express program provided by Applied Biosystems.

  • The resulting fragment (hCARΔcyt) was restriction endonuclease digested and subcloned into the EcoRI/SmaI site of the VA human CD2 mini-gene containing the human CD2 promoter and 3' LCR [ 16 ] . Automated sequencing (Applied Biosystems, Foster City, CA; University of Alabama at Birmingham Department of Microbiology sequencing facility) of the entire construct confirmed its accuracy.

  • The sequence and reading frame was validated by BigDye DNA sequencing (PE Applied Biosystems).

  • The quantification relative to the house keeping gene GAPDH was carried out in MicroAmp Optical 96-well reaction plates (PE Biosystems).

  • A specific exon 20 mutation (T790M) was also detected by length analysis of fluorescently labeled (FAM) PCR products on a capillary electrophoresis device (ABI 3100 Avant, Applied Biosystems, Foster City, California, United States), based on a new NlaIII restriction site created by the T790M mutation (2369 C→T), using the following primers: EGFR Ex20F, 5′-FAM- CTCCCTCCAGGAAGCCTACGTGAT-3′ and EGFR Ex20R 5′- TTTGCGATCTGCACACACCA-3′.


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