Example sentences for: biosystems

How can you use “biosystems” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • First strand cDNA was synthesized from 1 μg of total RNA in a 100 μl reaction volume using random hexamers as primer and Multiscribe reverse transcriptase (TaqMan Reverse Transcription Reagents Kit, PE Biosystems).

  • The sequences and necessary concentrations in the PCR reaction are outlined in Table 2. The PCR mixture included 0.625 U AmpliTaq Gold DNA polymerase in the 2× SYBR Green Master Mix (PE Biosystems), the required concentration of specific forward and reverse primers, 10 ng of cDNA template and 0.25 U AmpErase UNG (PE Biosystems) in a 25 μl reaction volume.

  • Quantitative RT-PCR assays were performed on the ABI Prism 7900HT sequence-detection system using the Taqman Reverse Transcription Kit (Applied Biosystems) and QuantiTech SYBRGreen PCR Kit (Qiagen) using a two-step reaction with protocols supplied by manufacturers.

  • Sequencing of each exon except exon 3 of GPC5 was performed using BODIPY dye primer chemistry [ 42 ] on an ABI 3700 DNA sequencer (Applied Biosystems, Foster City, CA, USA).

  • The amplification was carried out and analysed in the GeneAmp 5700 Sequence Detection System (PE Biosystems).


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