Example sentences for: biosciences

How can you use “biosciences” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Forty-eight hours after transfection, cells were lysed and membrane and supernatant fractions harvested as described previously [ 8 ] . 10 μg of membrane proteins and normalized volumes of the supernatants were resolved by SDS-polyacrylamide electrophoresis (10%), transferred to nitrocellulose, and probed with a monoclonal antibody to the EE epitope [ 34 ] . The antigen-antibody complexes were visualized with ECL chemiluminescence (Amersham Biosciences, Piscataway, NJ).

  • Purified bovine CL-derived microvascular endothelial cell populations were generated commercially by Cambrex Biosciences (BioWhittaker, Walkersville, MD) as described in detail [ 35 ] . In the present study, CLENDO cells from frozen aliquots (passage 3) were seeded (5,000 cells/cm 2) in 6- or 24-well plates or 10 cm culture dishes.

  • 1 mg/ml), 2 mM glutamine (JRH Biosciences, Lenexa, KS) and maintained at 37°C in a humidified atmosphere containing 5% CO 2 in air.

  • We studied the expression of circulating levels of cytokines IFN-γ, TNF-α, IL-10, IL-5, IL-4 and IL-2 in sera of patients using a new cytokine bead array system (BD Biosciences San Diego CA, USA).

  • Total RNA (2 μg) was reverse transcribed using SuperScript II Reverse transcriptase (Invitrogen) and random hexamers (Amersham Biosciences).


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