Example sentences for: biosciences

How can you use “biosciences” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Standard antibody-based enzyme-linked immunosorbent assay was used to measure supernatant concentrations of IP-10, MIG, IL-4, and IFN-γ according to the manufacturer's instructions (R&D Systems and BD Biosciences Pharmingen).

  • Forty-eight hours after transfection, cells were lysed and membrane and supernatant fractions harvested as described previously [ 8 ] . 10 μg of membrane proteins and normalized volumes of the supernatants were resolved by SDS-polyacrylamide electrophoresis (10%), transferred to nitrocellulose, and probed with a monoclonal antibody to the EE epitope [ 34 ] . The antigen-antibody complexes were visualized with ECL chemiluminescence (Amersham Biosciences, Piscataway, NJ).

  • All transcripts with a different restriction pattern were sequenced (primer sequences available upon request) using the Thermo Sequenase fluorescent labelled primer cycle sequencing kit (Amersham Biosciences) and analysed on an ALF-express automated DNA sequencer (Amersham Biosciences).

  • All antibodies were from BD Biosciences, San Jose, CA and analysis was performed using FACScan or FACScaliber cytometers (BD).

  • The levels of sulfated GAGs in lavage fluids were determined using a colorimetric dye-binding assay using 1,9-dimethylmethylene blue [ 23 ] . The levels of total nitrite in lavage fluids were measured with Nitric Oxide Assay kits (Calbiochem ®; Biosciences Inc, La Jolla, CA, USA).


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