Example sentences for: bioreagents

How can you use “bioreagents” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Polyclonal antibodies raised in rabbit against the sequence N 1829 KKKDDEVDRDAPSRKKAKE 1848 near the COOH-terminal domain of human IP 3 R1 were purchased from Affinity Bioreagents, Inc. (Golden, CO, cat #PA1-901).

  • 5% Tween 20, 1% milk) and incubated with primary antibody for 45 min at 37°C (1:1500 anti-eNOS mono [BD Transduction Laboratories, San Diego, CA, USA]; 1:1500 anti-nNOS poly [Zymed Laboratories, South San Francisco, CA, USA]; 1:200 anti-iNOS poly [Affinity Bioreagents, Golden, CO, USA]; and 1:1500 anti-β-actin mono [Sigma A5441; Sigma-Aldrich, St Louis, MO, USA]).

  • Antibody Reagents: All antibodies used in these experiments were purchased from Affinity Bioreagents, Golden, CO and all were diluted in M199 media to desired concentration.

  • Polyclonal antibodies raised in rabbit against a synthetic peptide with a sequence derived from the cytoplasmic, NH 2 -terminal domain of the rat IP 3 R2 protein (E 317 LNPDYRDAQNEGKTVRD 334 ), were also purchased from Affinity Bioreagents, Inc. (Golden, CO, cat #PA1-904).

  • However, iNOS protein was below the limit of detection following any level of hypoxic exposure (positive control was lipopolysaccharide-treated mouse spleen) and using several different antibodies (Affinity Bioreagents and BD Transduction Laboratories; Affinity Bioreagents blot shown), and nNOS was unchanged among the three experimental groups (positive control was mouse brain).


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