Example sentences for: bigt

How can you use “bigt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • BigT was then digested with PacI and AscI to release the entire floxed neo -tpA and EYFP assembly, and inserted into pROSA26PA digested with PacI and AscI.

  • To make the R26R-YFP targeting construct, the EYFP cDNA was excised from pEYFP-N1 with ApaI and NotI and inserted into BigT digested with ApaI and NotI.

  • BigT was then digested with PacI and AscI to release the entire floxed neo-tpA and CFP assembly, and inserted into pROSA26PA digested with PacI and AscI.

  • This was inserted into BigT digested with XhoI, Klenow filled, and then digested with NotI.

  • The XbaI site used for insertion into the ROSA26 genomic locus [ 4] was replaced by a linker (PacI, SwaI, AscI), so that it could be digested with PacI and AscI, and receive the bigT sequence.


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