Words similar to bigdye
Example sentences for: bigdye
How can you use “bigdye” in a sentence? Here are some example sentences to help you improve your vocabulary:
BAC insert ends were sequenced on an ABI377 automated sequencer (Applied Biosystems, Foster City, CA) using the ABI PRISM ®BigDye™ terminator cycle sequencing kit with T7 and SP6 sequencing primers.
DNA isolation was performed using a fast and inexpensive microwave-based protocol [ 84 ] . Sequencing reactions using the T3 (5') primer employed BigDye terminator chemistry (Applied Biosystems, Foster City, CA) and the cycle sequencing reactions were performed with 96 × 4-block thermocyclers (MJ Research, Waltham, MA).
Positive colonies were isolated and sequenced using a PRISM BigDye Terminator Cycle sequencing kit and an ABI 3700 automated DNA sequencer (Perkin-Elmer Applied Biosystems, Foster City, CA).
The 16S rDNA library was constructed as previously described [ 11 ] . The clones in the libraries were sequenced once from the 5' end using Bact27F (ABI PRISM BigDye Terminators on an ABI377XL sequencer (PE Applied Biosystems, Inc.; Foster City, CA) at the San Diego State University Microchemical Core Facility).
The sequence and reading frame was validated by BigDye DNA sequencing (PE Applied Biosystems).