Words similar to bigdye
Example sentences for: bigdye
How can you use “bigdye” in a sentence? Here are some example sentences to help you improve your vocabulary:
After Sephacryl HR-500 purification, amplicons were sequenced using the BigDye primer sequencing kit (PE Biosystems).
Purified plasmid DNA from transposon-bearing clones was sequenced using 2 μl ABI BigDye II Dye terminator mix (Applied Biosystems) in a 10-μl reaction.
All sequencing except SNP detection, which is detailed below, was done with the BigDye terminator sequencing kit (PE Biosystems, Foster City, CA) using LongRanger premixed gels (FMC/BioWhittaker) on an Applied Biosystems 377-XL96 DNA sequencer.
DNA isolation was performed using a fast and inexpensive microwave-based protocol [ 84 ] . Sequencing reactions using the T3 (5') primer employed BigDye terminator chemistry (Applied Biosystems, Foster City, CA) and the cycle sequencing reactions were performed with 96 × 4-block thermocyclers (MJ Research, Waltham, MA).
Positive colonies were isolated and sequenced using a PRISM BigDye Terminator Cycle sequencing kit and an ABI 3700 automated DNA sequencer (Perkin-Elmer Applied Biosystems, Foster City, CA).