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Example sentences for: bedford

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Using these membranes, filtration binding assays were performed according to previously described protocols [ 28 ] . To reduce nonspecific binding, 96-well filtration plates (fiberglass FB filters; Millipore, Bedford, USA) were pre-coated as suggested by the manufacturer with 0.5% polyethyleneimine + 0.1% BSA and then washed four times with 0.1% BSA.
Following electrophoresis, RNA was transferred to a nylon membrane (Hybond-N+, Millipore, Bedford, MA) according to the manufacturer's instructions, and hybridized with digoxigen-dUTP-labeled probe as described previously [ 4 ] . The primers used to generate the probe were: 5'-GGAATTCACCTGCTAATTACCTGA-3'and 5'-CGCGGATCCGTTTACACATAGTTATTGATAGAATCT-3'
The Centricon centrifugal ultrafiltration devices were from Amicon/Millipore, Bedford, MA.
Matrigel was from Becton-Dickinson (Bedford, MA) Athymic nude mice were purchased from Charles River (Wilmington, MA).
BG-1 infected clones were tested for tumorigenicity by injection of cells (10 6cells in 0.1cm 250% matrigel; Collaborative Biomedical Products, Bedford, MA, USA) into subcutaneous sites in 6-week-old athymic Ncr-nude mice (NCI Animal Program, Bethesda, MD, USA) that were ovariectomized at approximately 4 weeks of age.