Words similar to becton-

Example sentences for: becton-

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Then, 20 μl FITC-coupled anti-BrdU antibody (Becton-Dickinson) was added, followed by 30 minute incubation at room temperature in the dark.
Then 10 ul of fluorescein isothiocyanate (FITC) mouse anti-CD8 (Leu-2a) (Becton-Dickinson), and 10 ul peridinin chlorophyll (PerCP) mouse anti-CD3 (Leu-4) (Becton-Dickinson) were added, followed by incubation in the dark for 20 min at room temperature.
After another wash with 1 ml 0.5% Tween-20/1% BSA/PBS, nuclei were resuspended in 0.25 ml PBS containing 5 μg/ml Propidium Iodide, filtered through 1.2 ml Costar cluster tubes (Costar #4410), or 6 ml tubes with 35 μm strainer cap (Falcon 2235, Becton-Dickinson) in case of volumes greater than 0.5 ml.
The intensity of the green fluorescence of avidin-FITC-stained cells and the red fluorescence of PI stained cells was measured in the FACScan flow cytometer (Becton-Dickinson Immunocytometry, San Jose, CA, USA).
After washing with 1 ml of staining buffer, the cells were fixed with 600 ul of 0.5% PFA for approximately 1 day and then analyzed with a FACSCalibur flow cytometer (Becton-Dickinson) equipped with a FL4 channel.