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Example sentences for: becton

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Monoclonal antibodies were purchased from commercial vendors unless otherwise stated: CD3, IgG1; CD68, IgG1; CD34, IgG1; CD45, IgG1, D105, IgG1; and IgG2a controls (Dako Corporation, Carpinteria, CA, USA); anti-HLA-DR, IgG2a; CD14, IgG2b; CD34, IgG1 (Becton Dickinson, San Jose, CA, USA); anti-vimentin, IgG1; IgG2b control (Serotec, Kidlington, Oxfordshire, UK); anti-VCAM-1, IgG1; anti-αvβ3 (vitronectin receptor), IgG1 (Pharmingen, San Diego, CA, USA); anti-collagen-type-1, IgG1 (Sigma Diagnostics); anti-osteocalcin, IgG1 (ABOC-5021, Haematologic Technologies, Essex Junction, VT, USA) anti-IgG1.
Flow cytometric analyses for T cell and DC markers were performed on a FACScan flow cytometer (Becton Dickinson, San Jose, CA) in the University of California, Los Angeles, Jonsson Cancer Center Flow Cytometry Core Facility.
Allophycocyanin (APC)-conjugated CD14 (Becton, Dickinson and Co, San Jose, CA, USA) was added, and the cells were incubated for a further 20 min in the dark.
Indirect immunofluorescence was performed as reported previously [ 7 10 25 ] . HEp-2 cells were grown on eight-chamber vessel tissue culture slides (Becton Dickinson, Franklin Lakes, NJ, USA) and treated with 2 or 10 μM STS for up to 6 hours.
To quantitate biofilm formation, 20 μl of Difco crystal violet (Becton Dickinson, Sparks, Maryland) was added to each well and incubated at room temperature for 15 minutes.