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Example sentences for: becton

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Macrophage identity was confirmed by reactivity of cells with the mouse antihuman mAb, CD11c (Becton Dickinson, Mountain View, CA, USA).
Regan, University of Arizona) was used for immunocytochemical confirmation of protein expression in isolated thin limbs of Henle as described previously [ 30 ] . Tubules were positioned onto a glass microscope slide covered with a layer of Cell-Tak ®adhesive (Becton Dickinson, Bedford, MA).
The cells were filtered through a 70 μm cell strainer (Becton Dickinson) and pelleted by centrifugation in 50 ml conical tubes (maximum volume 25 ml) at 1200 rpm for 5 minutes.
Flow cytometric analysis was performed using a FACS Caliburflow cytometer utilizing CELLQuest software (Becton Dickinson, San Jose, CA, USA).
In addition, cells were stained with a combination of anti-CD3 PerCP, anti-CD8 FITC and anti CD4 PE (Becton Dickinson, San Jose, CA) . To assess their proliferative ability and to confirm the specificity of tetramer staining of ex vivo CD3+ CD8+ T-cells, lymphocytes were cultured at a concentration of 3 × 10 6/ml in RPMI enriched with 10% human serum with addition of 10 μg/ml of the appropriate peptide and 20 U/ml of IL-2 for 7 days.