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Example sentences for: becton

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In addition, cells were stained with a combination of anti-CD3 PerCP, anti-CD8 FITC and anti CD4 PE (Becton Dickinson, San Jose, CA) . To assess their proliferative ability and to confirm the specificity of tetramer staining of ex vivo CD3+ CD8+ T-cells, lymphocytes were cultured at a concentration of 3 × 10 6/ml in RPMI enriched with 10% human serum with addition of 10 μg/ml of the appropriate peptide and 20 U/ml of IL-2 for 7 days.
Cell culture plates, which are coated with poly- D-lysine (well diameter, 3.5 cm; Biocoat Poly- D-Lysine Cellware 6-Well Plates; Becton Dickinson Labware, Bedford, MA), were used as the solid surface.
For analytical flow cytometry, 10,000 events with forward and side scatter properties of lymphocytes were collected on a FACScan flow cytometer and analyzed using CellQuest ®software (Becton Dickinson; San Diego, CA).
The stained cells were fixed with 0.5 ml 1% paraformaldehyde and kept (covered with foil) at 4°C until further analysis on Flowcytometer (Becton and Dickinson, San Jose, CA) using Cell Quest software.
IgG antibody content was measured by enzyme-immunoassay employing a Fast Assay Screening Test System at room temperature [ 28 ] . Pegs protruding from a lid were placed over a 96-well plate or trough containing 14 ml of 10 μg/ml D-alanyl LTA in acetate buffer pH 5 for 2 h (Becton Dickinson, Lincoln Park, NJ).