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Example sentences for: basophils
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Neutrophils, eosinophils and basophils are at concentration ranges of 1800-7000/μl, 0-300/μl, and 0-100/μl, respectively, in normal adults [ 18 ] . Accordingly, the total cells yielded from these preparations are typically 3 × 10 7, 2 × 10 6, and 6 × 10 5leukocytes per preparation of neutrophils, eosinophils, or basophils, respectively.
For instance, 100 ml of blood yields only ~6 × 10 5basophils [ 17 ] . This number of basophils was not sufficient for studies in the classic chamber.
Design modifications have also been made that allow for pulsatile flow, a simulation of larger vessel blood flow, as well as lateral viewing that can give more detailed morphologic information [ 11 12 ] . Agarose-cast vessels and glass capillary flow chambers have also been described [ 12 14 15 ] . In spite of the large number of scientifically important observations that have been made with this technology, these chambers have at least two weaknesses: 1) Significant numbers of leukocytes are required, making study of rare leukocyte populations such as basophils or some lymphocyte subsets difficult, and 2) Large amounts of inhibitors are needed to study their effects, limiting the use of the parallel plate as a tool for drug discovery.
In order to assess the potential cellular requirements for an experiment, we assumed usage of standard isolation techniques of blood leukocytes with venipucture volumes that we and others employ for these experiments [ 6 13 16 17 ] , which are 30 ml, 100 ml, and 100 ml for neutrophils, eosinophils, and basophils, respectively.
In the past, it has not been feasible to study cell populations that were represented at less than 2% of peripheral blood leukocytes such as basophils, eosinophils, or some lymphocyte subsets.
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