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Example sentences for: baecs

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Addition of MTX to fura-2 -loaded BAECs, suspended in a cuvette in normal Ca 2+-containing buffer at 37°C, produced a time-dependent increase in [Ca 2+] i (Fig 1, upper panel ). Addition of either U73122 or U73343, the inactive analog, ~3 min before MTX produced a concentration-dependent inhibition of the change in [Ca 2+] i . U73343 exhibited a 3-fold greater potency compared to U73122 (Fig 1, insets ). Since U73343 has no significant effect on PLC over the concentration range employed [ 14 25 26 27 ] , these results suggest that inhibition of the MTX-induced response is independent of PLC.
BAECs on glass cover-slips were perfused with HBS at 37°C.
To test this hypothesis, BAECs were treated with vehicle alone (DMSO), U73343 alone, or MTX followed by U73343, and specific caspase-3 activity was determined after 48 hrs by the fluorescence substrate assay (Fig 7).
Specific caspase-3 activity, defined as the activity inhibitable by Ac-DEVD-CHO, was normalized to that of untreated BAECs.
MTX had no effect on BAECs incubated in Ca 2+-free buffer.