Example sentences for: autoradiography

How can you use “autoradiography” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Slides were then coated with emulsion, exposed, developed and stained in haematoxylin, as for in situ binding autoradiography.

  • Cyclin E variants in Cdk2 IP were detected by probing with HE12 primary antibody (PharMingen), followed by goat anti-mouse IgG and IgM (Jackson Immunoresearch) secondary antibody conjugated with horse radish peroxidase, and visualized by a chemiluminescent detection system (ECL, Amersham Pharmacia Biotechnology) and autoradiography as indicated above.

  • The radioactively labeled PCR products were separated on 6% denaturing polyacrylamide sequencing gels, and the alleles were visualized by autoradiography [ 6 ] . For the fluorescently labeled products, α- 32P-dCTP was omitted, fluorescent primers were used, and the products were analyzed in an ABI PrismTM 377 DNA Sequencer (Perkin Elmer) following the manufacturer's specifications.

  • The eIF-2 was precipitated with a polyclonal antibody against human eIF-2α (C-20) (Santa Cruz Biotech) in the presence of protein G-Sepharose (Amersham) for 1 h at 10°C, and then processed as described previously [ 21 ] . The immunoprecipitates were subjected to SDS-PAGE followed by autoradiography to examine eIF-2 phosphorylation.

  • Sections were used from in situ binding autoradiography experiments and were quantitated blind by one observer.


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