Words similar to astrocyte
Example sentences for: astrocyte
How can you use “astrocyte” in a sentence? Here are some example sentences to help you improve your vocabulary:
The RU486 effect is not likely to be mediated through PR in the Lifr -/- astrocyte precursors, since astrocytes have low levels of PR's that are only detectable in females [ 76 ] .
Cell lines were created by incorporation of retroviral vectors into the genome and expression of Tag from either the retroviral LTR (considered to be a strong promoter) or the herpes virus thymidine kinase promoter, internal to the viral LTR [ 1 ] . In our established NIH 3T3 cell line, Tag is expressed from the htk promoter at considerably lower levels than from the LTR for a considerable time after transduction [unpublished observations, and [ 11 ] ]. However, when a large number of htk-Tag astrocyte clones were checked 100 days after creation, the expression levels displayed a very large range, and thus we expect that this weak promoter is subject to positional effects on transcription or mutational events that affect gene expression (unpublished data).
However, based on unpublished observations and previous work (see Introduction,) [ 11 ] , we expected that the Tag expression-cell density profiles would look like that in Figure 3. To test this idea, we measured Tag expression by immunofluorescence flow cytometry (see Figure 4) in 6 Tag-immortalized mouse astrocyte lines plated at a range of cell densities and cultured for 2-3 days as previously described [ 15 ] . The upper densities ensured in most cases that the cells would be in either the "transition" or "plateau" phase for some of the measurements.
All of the lines contained GFAP, whereas NIH 3T3 cells treated with dibutyryl cAMP at the same level as the astrocyte lines did not.
Generation of astrocyte cell lines
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