Words similar to astrocyte

Example sentences for: astrocyte

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Generation of astrocyte cell lines
We have previously characterized clonal mouse astrocyte cell lines, immortalized by transduction of SV40 large T antigen expressed from the MoMuLV LTR [ 6 13 ] . Here, we compare variation in Tag levels in 3 of these lines and 3 additional lines (termed tkT lines), immortalized with Moloney Sarcoma Virus vectors expressing Tag from an internal herpes thymidine kinase (tk) promoter.
LIF is also responsible for increased GFAP expression [ 69 ] , while signaling through LIFR:gp130 is critical in fostering the differentiation of neuronal precursors into astrocytes [ 70 ] mediated through STAT3 [ 71 72 73 74 ] . Glial cells respond to GC, which, in turn, can affect transcriptional control of GFAP level either positively or negatively [ 74 ] . The present study shows that GC's can play a critical role in hindering astrocyte development in the absence of LIFR as seen through the partial abrogation of this defect following exposure to RU486 in Lifr -/- mice.
The astrocyte lineage of the LTR lines has been published [ 6 13 ] . The astrocyte lineage of the tkT lines was confirmed by the presence of the astrocyte-specific intermediate filament protein, GFAP [ 14 ] (Figure 1).
To test for astrocyte lineage, early passage cells were grown in dibutyryl cAMP (Sigma) to enhance GFAP expression.