Words similar to asn
Example sentences for: asn
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Molecular modeling suggested that this 'retention' motif - ExxHxR in the first and ExxNxR in the third membrane-spanning region - within all LHCPs and related proteins provides two ligands for Chl, an ion-pair between Glu (E) and Arg (R) and the sidechain of either His (H) or Asn (N).
However, in L1, Asn233 is 14 Å away from the modeled position of the substrate β-lactam carbonyl [ 37 ] . To test the role of Asn233 in substrate binding, the Asn was changed to a leucine (N233L) and to an aspartic acid (N233D), and these mutants were characterized by using metal analyses, CD spectroscopy, steady-state kinetics, and pre-steady state kinetic studies.
The substrate-binding model showed that Tyr228 in L1 was position-conserved with Asn233 in the other crystallographically characterized metallo-β-lactamases [ 37 42 44 45 46 ] . Spencer and coworkers postulated that Tyr228 is part of an oxyanion hole that interacts with the β-lactam carbonyl on substrate and helps to stabilize the putative tetrahedral intermediate formed during substrate turnover [ 37 ] . To test this hypothesis, Tyr228 was changed to an alanine and to a phenylalanine to afford the Y228A and Y228F mutants, respectively.
The fully edited rat 5-HT 2C receptor exhibits reduced agonist efficacy or ability to interact with G proteins compared to the unedited receptor (Ile157-Asn159-Ile161) in transfected cells [ 39 ] . Interestingly, 5-HT elicits a larger response (see Fig.
To examine a wider range of promoter and polymerase combinations, a promoter library was constructed in which positions -8 through -11 in the promoter were completely randomized; each of these nucleotides had previously been shown to be extremely important for the specificity of interactions with T7 RNA polymerase [ 6 7 9 11 ] . Conversely, residues Asn 748, Arg 756, and Gln 758 were known to form sequence specific contacts with this region of the promoter [ 2 ] . The promoter library was combined with the gene library in order to create approximately 51,200 (4 4× 20 3) combinations of promoters and polymerases.
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