Words similar to apai
Example sentences for: apai
How can you use “apai” in a sentence? Here are some example sentences to help you improve your vocabulary:
To make the R26R-YFP targeting construct, the EYFP cDNA was excised from pEYFP-N1 with ApaI and NotI and inserted into BigT digested with ApaI and NotI.
The resulting plasmid was digested with XbaI and ApaI to remove most of the 3' MCS (except for the terminal KpnI site), which was replaced with an AscI linker.
To generate pEGFP-COP1, pKS-COP1 was digested with ApaI and XbaI and the resulting fragment containing the MmCOP1 cDNA was subcloned into the same sites of the pEGFP I vector.
The resulting plasmid was called pBigT, and its MCS contains sites for the restriction enzymes NheI, SalI, AccI, XhoI, ApaI, SacII, NotI, SacI and BclI.
A MCS containing the restriction sites for XhoI, ApaI, SacII, NotI, SacI, EcoRV, and KpnI was synthesized and inserted downstream of the second loxP site by digesting the DNA with XhoI and KpnI and ligating in the MCS, which had been synthesized to have compatible cohesive ends.