Words similar to anti-rat

Example sentences for: anti-rat

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The proteins were transferred to nitrocellulose paper, and incubated at room temperature for 3 hours, or overnight, at 4°C, with mouse monoclonal anti-human Mcl-1 (1:1000), p38 MAP kinase, phosphoinositide 3 kinase (PI3K), or protein kinase B-alpha (PKB-α) (1:500) antibodies (Transduction Laboratories, San Diego, CA, USA and New England Biolabs, Inc., Beverly, MA, USA), or rabbit polyclonal anti-rat p44/42 MAP kinase (1:1000), or doubly phosphorylated anti-human Thr180/Tyr182 phospho-p38 MAP kinase (1:1000) antibodies (New England Biolabs, Inc.).
Figure 8Eis a control using the anti-rat Thy-1 antibody (not reacting with human tissues).
Secondary rabbit anti-rat horseradish peroxidase conjugated secondary antibody (Sigma) was added at a 1:2000 dilution for 2 h.
25%)-EDTA (1 mM) and trypan blue were purchased from GIBCO-BRL (Grand Island, NY); certified heat-inactivated fetal bovine serum (FBS) was obtained from Hyclone (Logan, UT); mouse anti-rat CD11b/c monoclonal antibody (OX-42) and rabbit anti-glutamate receptor (GluR4) polyclonal antibody were purchased from Pharmingen (San Diego, CA).
The monoclonal rat anti-human OPN antibody was optimized using the secondary biotinylated rabbit anti-rat antibody (1:600) and streptavidin/horseradish peroxidase (1:400; DAKO).