Words similar to anti-rabbit
Example sentences for: anti-rabbit
How can you use “anti-rabbit” in a sentence? Here are some example sentences to help you improve your vocabulary:
Fluorescein-labeled donkey anti-rabbit IgG and Cy5-labeled donkey anti-mouse IgG were purchased from Jackson ImmunoResearch.
Blots were first incubated overnight with gentle shaking at 4°C with rabbit anti-human TRAIL polyclonal antibodies (Cell Sciences, Inc., Norwood, MA) at a concentration of 0.2 μg/ml in Buffer A. Membranes were then washed the following day 3 times for 5 min in Buffer B with gentle shaking, then incubated for 1 h with gentle shaking at room temperature with horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (Amersham Biosciences Corp., Piscataway, NJ) diluted 1:5000 in Buffer A. Reactive proteins were visualized by enhanced chemiluminescence (Amersham, Arlington Heights, IL) following exposure to X-ray film and subsequent film development.
Blots were washed twice with TBS-T containing 0.5% milk, then incubated at room temperature for 2 hours in TBS-T containing 0.5% milk and 1:5000 dilutions of secondary Ab conjugated to peroxidase (for BMP-5, anti-goat IgG-peroxidase from Chemicon, Temecula CA; for Smad1, anti-rabbit Ig-peroxidase from Amersham, Piscataway, NJ).
Sections were washed and incubated for one hour with a 1:200 dilution of a secondary goat anti-rabbit Cy3-IgG antibody (Jackson Immuno-Research Laboratories, West Grove, PA), then washed in 1 × TBS for 2 × 10 min.
The membranes were then washed five times with TBS-Tween and were incubated with horseradish peroxidase-conjugated donkey anti-rabbit IgG (Bio-Rad, Hercules, CA), 1:5,000 dilution.
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