Words similar to anti-rabbit
Example sentences for: anti-rabbit
How can you use “anti-rabbit” in a sentence? Here are some example sentences to help you improve your vocabulary:
Membranes were blocked in 5% BLOT-QuickBlocker PBST buffer (plus 0.1% Tween-20), washed three times with PBST buffer (plus 0.1% Tween-20), incubated in primary antibody diluted in femto/TBST buffer in a tray for 90 min at room temperature (or overnight at 4°C), washed five times (as above), incubated in secondary antibody-HRP (horseradish peroxidase)-conjugate (goat anti-rabbit) as above, washed four times with femto/TBST buffer followed by transfer to detection buffer and detection reagent.
Secondary antibodies were a fluorescein-conjugated goat anti mouse IgG (Boehringer Mannheim, IN) used at a dilution of 1:1000 and a fluorescein-conjugated donkey anti-rabbit IgG (Chemicon, CA) used at a dilution of 1:1000.
Cellular 4-HNE protein adducts were detected by rabbit anti-4-HNE antibodies (Calbiochem, San Diego, CA, used at 1:250) followed by anti-rabbit secondary antibody labeled with FITC (Dako Corporation, Carpinteria, CA).
After adding 50 μ l goat anti-rabbit IgG bound to iron particles, incubation was continued in 0.5 ml for 1 day.
Cy3 conjugated donkey anti-mouse or anti-rabbit secondary antibodies (1:200, Sigma) in blocking solution were applied for 1 hour.