Example sentences for: anion-exchange

How can you use “anion-exchange” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • GAGs/polyanions purification was performed as follows: concentrated CM (15 ml) was dialyzed against buffer Q (50 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 6 M Urea, 0.5% CHAPS), filtered through a 0.4 micron membrane and its soluble components subjected to anion-exchange column chromatography using Q-Sepharose Fast Flow (1 ml bed volume).

  • The eluate from the SP column was then diluted 5-fold with buffer C (20 mM tris pH 8.5, 1 mM EDTA and 0.1% 2-mercaptoethanol) and subjected to anion-exchange chromatography on a BioScale Q column (2 ml from BioRad).

  • GAGs and polyanions in the CM were fractionated by anion-exchange column chromatography followed by PEDF-affinity column chromatography (Fig.

  • An FPLC-connected Mono Q HR 5/5 anion-exchange column (Pharmacia Biotech) equilibrated with 50 mM phosphate buffer (pH 7.0) containing 1 mM DTT was loaded with the concentrated *AroQ fraction.

  • Solubilized B. mori BBMV proteins were separated by Q Sepharose anion-exchange chromatography and all eluted fractions were tested for APN enzymatic activity.


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