Example sentences for: anion-exchange

How can you use “anion-exchange” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • An FPLC-connected Mono Q HR 5/5 anion-exchange column (Pharmacia Biotech) equilibrated with 50 mM phosphate buffer (pH 7.0) containing 1 mM DTT was loaded with the concentrated *AroQ fraction.

  • The sample was then loaded on a Q Sepharose HR 10/30 anion-exchange column.

  • A later study by these authors identified 120-kDa and 115-kDa APNs coeluting from an anion-exchange column that together yielded a Cry1Aa affinity of 53 nM [ 10 ] . These APNs eluted just prior to a 120 kDa APN with 7.6 nM affinity.

  • GAGs/polyanions purification was performed as follows: concentrated CM (15 ml) was dialyzed against buffer Q (50 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 6 M Urea, 0.5% CHAPS), filtered through a 0.4 micron membrane and its soluble components subjected to anion-exchange column chromatography using Q-Sepharose Fast Flow (1 ml bed volume).

  • The eluate from the SP column was then diluted 5-fold with buffer C (20 mM tris pH 8.5, 1 mM EDTA and 0.1% 2-mercaptoethanol) and subjected to anion-exchange chromatography on a BioScale Q column (2 ml from BioRad).

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