Words similar to anchorage-dependent
Example sentences for: anchorage-dependent
How can you use “anchorage-dependent” in a sentence? Here are some example sentences to help you improve your vocabulary:
As an alternative to the forced expression of ectopic PTEN in anchorage-independent, PTEN-negative tumor cells, our study has focused on the regulation of endogenous PTEN in anchorage-dependent mouse and human fibroblasts.
Previous studies by others had shown that the ectopic expression of PTEN in anchorage-independent tumor cells greatly reduced their ability to grow in soft agar [ 4 5 6 ] . In this latter situation, however, the forced expression of ectopic PTEN effectively impairs cellular proliferation in general, even under two-dimensional culture conditions where the cells are attached to substratum [ 4 6 7 8 9 10 18 33 44 ] . It was therefore difficult to discern from these experiments how effectively and selectively PTEN participates in anchorage-dependent growth control.
In light of the well-established growth-inhibitory effects exerted by increased levels of this phosphatase, it is reasonable to conclude that this activation of PTEN significantly contributes to the anchorage-dependent phenotype, i.e., to the inhibition of cell proliferation after detachment from matrix.
In this current study, we investigated the response of the PTEN tumor suppressor to changes in cell-matrix interactions of anchorage-dependent human and mouse fibroblast cells.
This protein acts as inhibitor of cyclin-dependent kinases (the "cell cycle engine" [ 50 ] ), and its elevated expression has been consistently demonstrated in different cell types after the disruption of cell-matrix interactions (compare Figure 1and [ 31 32 51 52 ] ). Furthermore, p27 Kip1is an established target of PTEN signaling, i.e., its activity has been found increased after the forced expression of exogenous PTEN [ 53 54 55 56 ] . In combination with the data presented in this manuscript, it therefore appears that PTEN contributes to anchorage-dependent growth control by a two-fold approach: the dephosphorylation of the signaling molecule FAK in combination with the stimulation of the cell cycle inhibitor p27 Kip1.
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