Words similar to anchorage-dependent
Example sentences for: anchorage-dependent
How can you use “anchorage-dependent” in a sentence? Here are some example sentences to help you improve your vocabulary:
The murine cell line 10T1/2 and the human cell line MDAH, both of which are anchorage-dependent fibroblasts, were detached from their tissue culture dishes and cultured further on HEMA-coated plates.
In this current study, we investigated the response of the PTEN tumor suppressor to changes in cell-matrix interactions of anchorage-dependent human and mouse fibroblast cells.
As expected in the case of anchorage-dependent cells, the activity of cyclin-dependent kinases was strongly reduced and cell proliferation was inhibited under these suspension culture conditions (not shown).
Previous studies by others had shown that the ectopic expression of PTEN in anchorage-independent tumor cells greatly reduced their ability to grow in soft agar [ 4 5 6 ] . In this latter situation, however, the forced expression of ectopic PTEN effectively impairs cellular proliferation in general, even under two-dimensional culture conditions where the cells are attached to substratum [ 4 6 7 8 9 10 18 33 44 ] . It was therefore difficult to discern from these experiments how effectively and selectively PTEN participates in anchorage-dependent growth control.
Collectively, they have established that the expression of cyclin A and cyclin D, in combination with the activity of the cyclin-dependent kinase inhibitors p21 Cip1and p27 Kip1, is a crucial determinant of anchorage-dependent cell growth culture [ 27 28 29 30 31 32 ] . However, while the above elements clearly are essential executioners of cell cycle progression, it is conceivable that other elements, directly or indirectly, might be involved in anchorage-dependent growth control as well.
Loading...