Example sentences for: amperase

How can you use “amperase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Each 25 μl PCR mix contained 7.875 μl Sybr ®Green Master Mix (1X Sybr ®Green Buffer, 3 mM MgCl 2 , 200 nM dNTP blend, 0.6275 U Amplitaq Gold ®, 0.25 U AmpErase ®UNG), 200-400 nM forward and reverse primers and approximately 5 ng template.

  • Standard reaction volume was 10 μl and contained 1X QuantiTect SYBR Green PCR master mix (Qiagen, Valencia, CA), 0.002 U AmpErase UNG enzyme (PE Biosystems, Forest City, CA), 0.7 μl of cDNA template and 100-500 nM of oligonucleotide primers.

  • The sequences and necessary concentrations in the PCR reaction are outlined in Table 2. The PCR mixture included 0.625 U AmpliTaq Gold DNA polymerase in the 2× SYBR Green Master Mix (PE Biosystems), the required concentration of specific forward and reverse primers, 10 ng of cDNA template and 0.25 U AmpErase UNG (PE Biosystems) in a 25 μl reaction volume.

  • The PCR reaction was performed in a final volume of 50 μl using TaqMan Universal PCR Master Mix containing AmpliTaq Gold RDNA Polymerase, AmpErase RUNG, dNTPs (with dUTP), Passive Reference 1, optimized buffer components (proprietary formulation) and 1 μl of cDNA template.


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