Words similar to ampcp
Example sentences for: ampcp
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These data indicate that VO 2+-AMPCP was released from the conformation corresponding to species D2; however, the conformation corresponding to species D1 still had bound VO 2+-AMPCP.
In order to distinguish species D1 from D2, the sample with VO 2+-AMPCP bound to sCD39 was thawed and incubated at room temperature for 30 minutes, and the VO 2+EPR spectrum was collected again.
Figure 3ashows the parallel features of the EPR spectrum of sCD39 bound VO 2+-AMPCP.
Incubation of sCD39 with VO 2+-AMPCP at room temperature resulted in a dramatic decrease of the intensity of species D2, while the signal intensity of species D1 remained unchanged.
However, intact CD39 does not release intermediate ADP during ATP hydrolysis, suggesting that there is only one ADP binding site on each CD39 monomer in the intact protein [ 2 25 ] . The two EPR species observed with VO 2+-AMPCP probably correspond to the two different conformations of bound ADP at the same catalytic site on sCD39.
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