Example sentences for: ambion

How can you use “ambion” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Standard RNA was made for all assays by the T7 polymerase method (Ambion, Austin, TX), using total RNA isolated from the mouse heart.

  • After RNA amplification, the DNA template was removed by incubation of the reaction with 4 units of RNase-free DNase I (Ambion) at 37°C for 15 min. aRNA purification was achieved by Rneasy Mini Kit (Qiagen).

  • The resolved probes were detected using BrightStar kit (Ambion) and exposed to films.

  • The first round combined reverse transcription using oligo-dT (15)-T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGC-3') primer and template-switch primer (5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3') (Clontech, Palo Alto, CA) catalyzed by the Superscript-11 reverse transcriptase (Gibco-BRL) and Advantage cDNA polymerase (Clontech) for single- and double-strand cDNA synthesis respectively, with in vitro transcription (Megascript kit, Ambion, Austin, TX).

  • Each double-stranded cDNA sample was purified by sequential phenol/chloroform extraction (Ambion, Austin, TX) and adsorption to silica (Qiaquick™ kit, Qiagen, Hilden, Germany) according to manufacturers' instructions.


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