Example sentences for: alphoid

How can you use “alphoid” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The probes were labeled for hybridization by PCR using Alu and alphoid DNA monomers as templates and repeat-specific primers (Table 2).

  • The third vector, pARS-ALF, was designed for cloning human alphoid DNA arrays.

  • Approximately 15% of the isolates contained alphoid DNA arrays interrupted with nonalphoid DNA sequences, including Alu and LINE repeats.

  • When centromeric DNA is digested by a restriction endonuclease, the resulting fragments are usually either too small or too large for efficient cloning in bacterial artificial chromosomes [ 22 ] . In recent work we reported isolation of human centromeric DNA using a TAR vector lacking ARS element [ 28 ] . While the isolation was selective, a standard TAR cloning technique does not allow to recover a significant fraction of alphoid DNA arrays that does not contain ARS . In our present study, using the new TAR cloning strategy clones of human alphoid DNA up to ~ 210 kb in length were isolated with high selectivity.

  • The relatively large sizes of the inserts suggest that genomic alphoid DNA fragments recombine with targeting hooks in the vector by sequences located at DNA fragment ends, rather than by internal sequences.


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