Example sentences for: alone

How can you use “alone” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Top 10 F' E. coli (Stratagene) were transformed with 1 μL of the transposition reaction and plated on LB agar with either ampicillin (100 μg/mL) and kanamycin (50 μg/mL) to recover transposed clones, or ampicillin (100 μg/mL) alone to establish the transposition efficiency.

  • In this experiment, a Wg plasmid activated the luciferase reporter only 12-fold above the vector control; wild-type DFz2 plasmid alone stimulated luciferase levels 7-fold, but neither deletion mutant of DFz2 had any effect, consistent with an earlier report that DFz2 with a truncated carboxyterminus fails to induce the expression of Xnr/Siamois [ 21 ] . However, DFz2ΔC stimulated signaling over 700-fold when delivered in concert with Wg, a synergistic effect nearly as strong as observed with wild type DFz2 (nearly 1700-fold in this experiment).

  • In the last four months alone, the King family has:

  • It is difficult to interpret transcriptional profiling data alone, since dimerization, post-translational modifications, relative quantities of co-activators and co-repressors, and the formation of multiple transcriptional complexes binding to common promoter regions ultimately determines the binding activation potential of transcription factors.

  • The majority of peripheral blood T cells from RA patients secreted IL-2 alone, whereas fewer cells secreted IFN-γ alone or in combination with IL-2.


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