Words similar to aliquoted

Example sentences for: aliquoted

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The lysates were centrifuged at 4000 × g for 15 minutes, then aliquoted and stored at -20°C prior to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western analysis as previously described [ 12 ] . Protein concentrations of the cell lysates were determined using the Bio-Rad Protein Assay (Hercules, CA), and sample volumes adjusted so that 5-10 μg of total protein were loaded into wells of a discontinuous (12% separating, 4% stacking) polyacrylamide gel.
Human cytomegalovirus (CMV) lysate was obtained from Advanced Biotechnologies (Columbia, MD), aliquoted and stored at -80°C, and used at the doses indicated in the figures.
For Figure 4, separated cells were then aliquoted and treated (or not) with 5 μg/ml 5A6 anti-CD81 mAb ( [ 11 ] , a gift of Shoshana Levy, Stanford University) for one hour on ice.
The red blood cell suspension was then aliquoted into cryovials and frozen at -40°C until transported in the frozen state to the University of New Mexico School of Medicine for analysis.
Vectors were purified using two consecutive CsCl gradient ultracentrifugation, dialyzed at 4°C against sterile virus buffer, aliquoted, and stored at -80°C until use.