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Example sentences for: ad-

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2, infection of MCF-7 cells with Ad-GFP/Rz-IGF2R resulted in positive expression of the ribozyme as determined by RT-PCR.
In addition, after infection with Ad-GFP or Ad-GFP/Rz-IGF2R and treatment with TNF, cell viability was assessed using the MTT assay Kit (Roche Molecular Biochemicals) and cell apoptosis was determined using the Cell Death Detection ELISA Kit assay (Roche Molecular Biochemicals) according to the manufacturer's protocol.
There were significantly more apoptotic cells in control cultures than in cultures expressing the Ad-GFP/IGF2R-Rz.
There was no significant difference in the level of M6P/IGF2R mRNA between Ad-GFP-infected cells and uninfected cells (data not shown); indicating that infection with the adenovirus itself did not alter the endogenous M6P/IGF2R mRNA level.
To examine the ability of the ribozyme to reduce levels of M6P/IGF2R mRNA in MCF-7 cells, total RNA was extracted from cells infected with Ad-GFP/IGF2R-Rz or Ad-GFP, and subjected to RT-PCR using M6P/IGF2R-specific primers.