Example sentences for: acrylamide

How can you use “acrylamide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Depending on whether the sense or anti-sense strand was to be sequenced, either the forward or reverse primer was modified with a 5' acrydite to covalently attach the PCR product to the acrylamide matrix (see below).

  • The reaction mixtures were electrophoresed on a 6 % non-denaturing polyacrylamide gel (acrylamide: bisacrylamide, 30: 0.8) containing 5 % glycerol at 180 V in TAE buffer (1 mM DEAE, 3.3 mM sodium acetate and 6.7 mM Tris, pH 7.5).

  • The radiolabeled PCR products that reflected the HCDR3 lengths of various B-cell clones in the cell suspension were electrophoresed through a 6% denaturing acrylamide sequencing gel for approximately 1.5 h.

  • The radiolabeled PCR products were digested with Alu I or Hpa II, denatured and electrophoresed for 10-16 h at 500 V on non-denaturing 6% acrylamide gels at room temperature [ 24 ] . For RFLP analysis, PCR products were digested with Mbo II and analyzed by electrophoresis on non-denaturing 6% acrylamide gels at 500 V for 3-4 hours.

  • Sequencing reactions were resolved on 48 cm polyacrylamide gels (4%, 19:1 acrylamide:bisacrylamide, Bio-Rad Laboratories) using an ABI model 373 fluorescence sequencer (Applied Biosystems, Inc.).


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