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Example sentences for: acidophilum

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Thermoplasma acidophilum is among sixteen archaea for which inteins have been reported to date (Intein database http://www.neb.com/inteins/int_reg.html [ 18 ] ). Members of the genus Thermoplasma lack cell walls and possess a cytoskeleton.
Here we present data on the cloning and expression of the T. acidophilum A-ATPase A subunit in E. coli, and we discuss implications for the location, propagation and distribution of inteins among organisms.
They live in hot and acidic environments, and are often found adhering to sulfur particles [ 19 ] . T. acidophilum grows optimally at 59°C and at an external pH between 1-2 [ 20 ] . A cytoplasmic pH of 5.5 has been measured indirectly [ 21 ] .
This activity does not depend on the physicochemical conditions in the T. acidophilum cytoplasm.
Multiple sequence alignments of diverse intein sequences identified eight motifs composed of moderately conserved residues [ 18 28 ] . The A-ATPase A subunit of the Thermoplasma and Pyrococcus intein multiple sequence alignment (with manual modification) is shown in figure 1. The T. acidophilum intein (173 amino acids long) is among the shortest inteins known, and the alignment with other inteins reveals the absence of sequences homologous to the typical endonuclease motifs.