Words similar to acidophilum
Example sentences for: acidophilum
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Multiple sequence alignments of diverse intein sequences identified eight motifs composed of moderately conserved residues [ 18 28 ] . The A-ATPase A subunit of the Thermoplasma and Pyrococcus intein multiple sequence alignment (with manual modification) is shown in figure 1. The T. acidophilum intein (173 amino acids long) is among the shortest inteins known, and the alignment with other inteins reveals the absence of sequences homologous to the typical endonuclease motifs.
1) shows that the T. acidophilum and T. volcanium inteins do not contain an endonuclease domain.
We selected eight genomes from archaea: Archaeoglobus fulgidus (AF), Aeropyrum pernix K1 (AP), Halobacterium sp. (HS), Methanococcus jannaschii (MJ), Methanobacterium thermoautotrophicum (MT), Pyrococcus abyssi (PA), Pyrococcus horikoshii (PH), and Thermoplasma acidophilum (TA); 14 genomes from bacteria: Aquifex aeolicus (AA), Borrelia burgdorferi (BB), Bacillus subtilis (BS), Chlamydia pneumoniae strain AR39 (CP), Chlamydia trachomatis (CT), E. coli strain K12 (EC), Haemophilus influenzae (HI), Helicobacter pylori strain 26695 (HP), Mycobacterium tuberculosis (MyTu), Mycoplasma genitalium (MG), Mycoplasma pneumoniae (MP), Rickettsia prowazekii (RP), Synechocystis sp. (SS), and Treponema pallidum (TP); four genomes from eukaryotes: Saccharomyces cerevisiae (SC), D. melanogaster (DM), C. elegans (CE), and Arabidopsis thaliana (AT).
The gene encoding the T. acidophilum A-ATPase A subunit was cloned into the expression vector pET-11a (Stratagene) and transformed into E. coli Bl21(DE3) and E. coli Bl21-CodonPlus(DE3)-RIL strain for protein expression.
This activity does not depend on the physicochemical conditions in the T. acidophilum cytoplasm.
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