Example sentences for: acidophilum

How can you use “acidophilum” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Despite the chemical and physical differences between the E. coli cytoplasm and the environment in which the T. acidophilum intein is functioning in vivo, we found no indications that self-splicing of the intein was inefficient in E. coli.

  • Multiple sequence alignments of diverse intein sequences identified eight motifs composed of moderately conserved residues [ 18 28 ] . The A-ATPase A subunit of the Thermoplasma and Pyrococcus intein multiple sequence alignment (with manual modification) is shown in figure 1. The T. acidophilum intein (173 amino acids long) is among the shortest inteins known, and the alignment with other inteins reveals the absence of sequences homologous to the typical endonuclease motifs.

  • When E. coli Bl21(DE3), a strain that did not express additional rare tRNAs, was transformed with the cloned T. acidophilum ATPase A subunit, no additional protein bands were observed in extracts of induced cells (not shown).

  • The T. acidophilum intein retains efficient self-splicing activity when expressed in E. coli.

  • In contrast, the T. acidophilum subunit is expressed as a soluble protein in the E. coli cytoplasm.


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