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Example sentences for: acidified

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To examine whether mactinin was present in immune complexes in rheumatoid arthritis fluid, some samples were acidified by dialysis against 0.1 M sodium acetate, pH 4.1, to dissociate complexes [ 18 ] . The acidified samples were first fractionated on an 800 ml G-75 Sephadex (Pharmacia, Piscataway, NJ) size-exclusion column.
To dissociate immune complexes, an aliquot of a rheumatoid arthritis fluid sample was acidified and the proteins were fractionated on a C-4 column.
A very recent communication of M. paratuberculosis interactions with macrophages was published during the course of our experiments [ 17 ] . This comprehensive study showed that M. paratuberculosis -containing vacuoles were mildly acidified (pH 6.3) as compared to latex beads (pH 5.2).
It is known that, when alkaline aqueous UCB is acidified to neutral or low pH's, "Usually a colloid suspension of bilirubin is formed and the solution remains clear, as observed by the naked eye, thus inviting an erroneous interpretation" [ 11 ] . Such systems, which simulate true solutions, often show sedimentation on centrifugation [ 12 13 14 15 16 ] . We here report that sedimentation of UCB from apparently clear solutions can likewise be extensive when UCB in DMSO is diluted with aqueous buffers to final mole fractions (N) of DMSO = 0.025, 0.086 and 0.31, corresponding to 9, 27, and 64 vol% of DMSO.
For most experiments, the mice were fed NIH31 (6 % fat) chow ad libitum , and their water was acidified to pH 2.8 to 3.2.