Words similar to acidified
Example sentences for: acidified
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The purple formazan product indicative of viable cells was then dissolved by adding 250 μl acidified isopropanol (95% isopropanol; 5% 2N HCl), and 200 μl aliquots were collected.
To examine whether mactinin was present in immune complexes in rheumatoid arthritis fluid, some samples were acidified by dialysis against 0.1 M sodium acetate, pH 4.1, to dissociate complexes [ 18 ] . The acidified samples were first fractionated on an 800 ml G-75 Sephadex (Pharmacia, Piscataway, NJ) size-exclusion column.
It is known that, when alkaline aqueous UCB is acidified to neutral or low pH's, "Usually a colloid suspension of bilirubin is formed and the solution remains clear, as observed by the naked eye, thus inviting an erroneous interpretation" [ 11 ] . Such systems, which simulate true solutions, often show sedimentation on centrifugation [ 12 13 14 15 16 ] . We here report that sedimentation of UCB from apparently clear solutions can likewise be extensive when UCB in DMSO is diluted with aqueous buffers to final mole fractions (N) of DMSO = 0.025, 0.086 and 0.31, corresponding to 9, 27, and 64 vol% of DMSO.
To dissociate immune complexes, an aliquot of a rheumatoid arthritis fluid sample was acidified and the proteins were fractionated on a C-4 column.
The animal room was maintained on a 14L:10D cycle, and room temperature was 25-28°C; food (Fox Reproduction Food; Milk Specialties Co., New Holstein, WI) and acidified water (pH= 4.2) were provided ad libitum . Males (n = 6 proven breeders) and females were caged individually and held on different shelving racks, separated by at least 2 m.
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