Words similar to abi
Example sentences for: abi
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Quantitative PCR can be performed on the DHPLC system, the ABI 7700 Sequence Detector, the Bio-Rad iCycler or by standard densitometric procedures.
A number of Arabidopsis sugar-response mutants have been isolated that, unlike wild-type plants, are able to develop substantial shoot systems in the presence of 0.27 to 0.33 M sucrose or glucose [ 46 47 48 49 50 ] . Analysis of a representative mutant of this type, the sis5-1/abi4-101 mutant [ 46 ] , reveals that it is resistant to the inhibitory effects of exogenous glucose on seed storage lipid breakdown.
DNA was sequenced using the ABI PRISM BigDye Terminator Cycle Sequencing (PE Applied Biosystems).
The annealing temperatures for each primer pair are listed in Table 1. Sequencing was with dye-labeled terminators (ABI 377 Sequencer).
Several groups of Arabidopsis mutants that are resistant to the inhibitory effects of high sugar concentrations have been isolated [ 46 47 48 49 50 ] . Characterization of these mutants reveals that the sis5 [ 46 ] , sun6 [ 50 ] , gin6 [ 49 ] and isi3 [ 45 ] mutants are allelic to the abscisic acid insensitive mutant abi4 [ 51 ] and the sis4 [ 46 ] , isi4 [ 45 ] and gin1 [ 45 ] mutants are allelic to the abscisic acid biosynthesis mutant aba2 [ 52 ] . In addition, the ethylene constitutive response mutant ctr1-1 [ 53 ] , as well as the ethylene-overproduction mutant eto1 [ 54 ] , display sugar-resistant phenotypes [ 48 ] . Conversely, the sugar-response mutant sis1 exhibits an ethylene constitutive response phenotype and is allelic to ctr1-1 [ 47 ] . The prl1 mutant, which exhibits increased sensitivity to exogenous sugars [ 55 56 ] , carries a mutation in a gene that encodes a WD protein that interacts with SNF1 protein kinases [ 57 ] .
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