Words similar to abasic

Example sentences for: abasic

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The importance of such bonds for activity was shown in other work, where huAPE1's binding to the DNA backbone is only inhibited by ethylation of the phosphates two and three positions 3' to an abasic site [ 18 ] . Mutation of R177A, at the end of Molego 6, that binds to this region and to the bases opposite the AP-site had enhanced activity [ 11 ] , while mutations at W280 (Molego 11) and F266 (Molego 10) [ 33 ] reduce activity and, in the latter case, substrate selectivity.
This generates the necessary 3' hydroxyl group for DNA polymerases (pol β, δ or ε, in eukaryotes) to insert the correct nucleotide in later steps in the base excision repair pathway (BER-pathway) [ 7 8 ] . Recent crystal structures of huAPE1 complexed with DNA containing an abasic site [ 9 10 11 ] , combined with sequence analysis and site-directed mutagenesis, have defined the residues that participate in metal ion based cleavage of the phosphate backbone of the DNA [ 12 13 14 15 16 17 ] .
We have used abasic residues at the 5' ends of P2 primers in order to reduce these artifacts.
Of the four protein areas that bind to the DNA 3' of the abasic site, mutating F266 (molego 10) or W280 (middle of molego 11) decreases APE activity [ 33 ] . The F266 mutation is particularly interesting, as the mutants at this position had reduced substrate specificity and enhanced 3'-exonuclease activity.
The abasic sugar-phosphate backbone is then cleaved by an apurinic/apyrimidinic endonuclease or lyase.