Example sentences for: abasic

How can you use “abasic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Both enzymes cleave only one DNA strand in a duplex and bacterial Xth cleaves ssDNA containing an abasic site [ 42 ] . The additional contacts huAPE1, compared to DNase 1 (Figure 6), makes 3' to the damage site and to the opposite strand lower its turnover rate and its potential to cleave normal DNA.

  • The abasic sugar-phosphate backbone is then cleaved by an apurinic/apyrimidinic endonuclease or lyase.

  • APEs are essential for mammalian cell growth and bacterial survival in the presence of ionizing radiation and DNA mutagens [ 6 ] . They initiate repair of an abasic DNA site by cleaving the phosphodiester backbone 5' of the phosphodeoxyribose.

  • Of the four protein areas that bind to the DNA 3' of the abasic site, mutating F266 (molego 10) or W280 (middle of molego 11) decreases APE activity [ 33 ] . The F266 mutation is particularly interesting, as the mutants at this position had reduced substrate specificity and enhanced 3'-exonuclease activity.

  • The importance of such bonds for activity was shown in other work, where huAPE1's binding to the DNA backbone is only inhibited by ethylation of the phosphates two and three positions 3' to an abasic site [ 18 ] . Mutation of R177A, at the end of Molego 6, that binds to this region and to the bases opposite the AP-site had enhanced activity [ 11 ] , while mutations at W280 (Molego 11) and F266 (Molego 10) [ 33 ] reduce activity and, in the latter case, substrate selectivity.


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