Example sentences for: abasic

How can you use “abasic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Although motif 12 contains several highly conserved residues that, according to mutagenesis results (Table 1) contribute to APE1 activity, only His309 is very close to the abasic site in the DNA.

  • An alanine substitution mutant, N226A, of a conserved residue at the end of molego 7a that forms a hydrogen bond with the second phosphate group downstream of the abasic site, had enhanced APE activity but increased Km and Kd values, similar to an alanine mutant of R177, which binds to the same site, reported previously [ 34 ] . A combination of the two mutants, N226A and R177A, substantially reduced the ability of APE1 to bind to DNA containing an abasic site (Izumi et al.

  • The 3' approach to the DNA [ 34 ] and the wide area covered by the protein on both sides of the abasic site [ 14 ] are both consistent with the need to hold the product until the correct polymerase moves in 5' to 3' to complete the repair [ 25 ] . This implies that the mammalian enzyme has evolved to be processive, to facilitate more efficient functioning of the overall BER pathway, and may not be optimized for simple catalysis.

  • Of the four protein areas that bind to the DNA 3' of the abasic site, mutating F266 (molego 10) or W280 (middle of molego 11) decreases APE activity [ 33 ] . The F266 mutation is particularly interesting, as the mutants at this position had reduced substrate specificity and enhanced 3'-exonuclease activity.

  • P2s have two abasic residues at the 5' end to reduce primer-dimer formation (unpublished data).


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