Example sentences for: aa-d

How can you use “aa-d” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For each sample to be analyzed by microarray, 4 μg amplified RNA were primed with 5 μg random hexamers and 5 μg oligo dT and reverse-transcribed, incorporating aa-dUTP, as described at http://www.microarrays.org.

  • Reverse transcription was started by adding dNTPs to a final concentration of 1 mM dATP and 500 μM each: dCTP, dGTP, dTTP and 5-(3-aminoallyl)-2'-deoxyuridine-5'-triophosphate, (aa-dUTP) (Sigma), with 150 units of StrataScript (Stratagene, La Jolla, CA).

  • The resulting aa-dUTP-containing cDNA was coupled to CyScribe Cy3 or Cy5 (Amersham, Piscataway, NJ) monofunctional dye in the presence of 0.1 M NaHCO 3 pH 9.0.

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