Words similar to protease
Example sentences for: protease
How can you use “protease” in a sentence? Here are some example sentences to help you improve your vocabulary:
After incubation in the presence of cell-death-inducing reagents, Jurkat cells were centrifuged at 200 × g for 30 min, followed by one wash at 1000 × g for 10 min in PBS containing Complete Protease Inhibitor cocktail (Roche, Mannheim, Germany).
Cells were washed twice with PBS and lysed with RIPA buffer (1% Triton X-100, 1% NaDOC, 0.1% SDS, 158 mM NaCl, 10 mM Tris pH 7.2, 5 mM NaEDTA and 1% protease inhibitors, added just before use) (Sigma, St. Louis, MO).
5 μg total protein) were incubated in polypropylene tubes with 2 nM [ 3H]-Hh-Ag 1.5 in the presence or absence of 2 μM competitor in binding buffer (50 mM Tris pH 7.5, 5 mM MgCl, 1 mM EDTA, 0.1 % bovine serum albumin) plus EDTA-free protease inhibitor cocktail (Roche) in a final volume of 250 μl for 1-26 hours at 37°C.
5, 250 mM sucrose with protease inhibitors PMSF, Leupeptin, Aprotinin) by a dounce homogenizer times10 strokes.
Importantly, it looks likely that protease inhibitors such as EPI1 are present in other