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Example sentences for: perkinelmer

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30–2001) supplemented with 10% fetal calf serum, 10 units/ml penicillin, and 10 μg/ml streptomycin) at 37 °C and 5% CO 2 . For viability studies, cells were seeded in complete growth medium in black 96-well clear bottom ViewPlates (PerkinElmer, Wellesley, Massachusetts, United States) at a density of 5,000 (H1975 and H2030) or 7,500 cells per well (H3255).
The blot was washed once for 5 minutes in PBS-T, incubated with goat-anti-rabbit conjugated HRP (1:10000 dilution) for 1 hour, washed for 5 minutes in PBS-T and developed with Western Lightning Chemiluminescence Reagent Plus (PerkinElmer Life Sciences, Inc.
After 1 hr incubation at RT with 50 μl/well of biotinilated secondary antibodies, plates were developed with Eu-labelled Streptavidin (PerkinElmer Life Sciences, Boston, MA) followed by Enhancement solution (PerkinElmer Life Sciences) and read in a 1234 Delfia Fluorometer (PerkinElmer Life Sciences).
Calcein fluorescence (Ex, 485 nm; Em, 535 nM) was detected immediately using a Victor V multi-label plate reader (PerkinElmer).
The samples were vortexed and were incubated at 100°C for 30 minutes, and the absorbance at 532 nm was measured using a PerkinElmer Lamba 3B spectrophotometer (PerkinElmer, Wellesley, MA, USA).