Example sentences for: perkin-

How can you use “perkin-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The reactions were carried out in the Universal PCR master mix, containing MgCl 2 , dNTP and DNA polymerase AmpliTaq Gold™ (Perkin-Elmer) in a total volume of 50 μl.

  • Genomic DNA and total RNA were extracted from fibroblast cultures established from skin biopsies as described [ 14 ] . Long-range RT-PCR amplification of FBN1 cDNA fragments and restriction enzyme digestions were performed as previously reported [ 8 ] . Fragments migrating abnormally in agarose gel electrophoresis were excised, purified with the QIAquick Gel Extraction Kit (QIAGEN) and directly sequenced with fluorescent terminators on an ABI Prism 377 DNA sequencer (Perkin-Elmer).

  • PCR amplification was performed, with final concentration of the digest equivalent to approximately 100 ng of the DNA, 1 × PCR bufferII (Perkin-Elmer, Foster City, CA, USA), 2.5 mmol/l MgCl 2 , and amplitaq gold 1.25 units.

  • Dual color fluorescence was detected using an ABI Prism 7700 Sequence Detection System (Perkin-Elmer Corp., Foster City, CA).

  • Aliquots of cDNA target template were diluted serially and mixed with 300 nM of primers and 200 nM of fluorogenic probe (Perkin-Elmer, Foster City, CA).


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