Example sentences for: pbd-lemaf

How can you use “pbd-lemaf” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1Bshows the quantification of β-Galactosidase activity in the pBD-LeMAF1/pAD-LeFPP strain.

  • Lanes one and two are the positive and negative control provided with the kit, respectively, lane three shows the activity of the pBD-LeMAF1/pAD-LeFPP strain and lane 4 the activity of the pBD-GAL4/pAD-LeFFP strain.

  • A yeast two-hybrid screen was performed to identify interaction partners of the tomato nuclear envelope-associated protein LeMAF1 [ 36 ] . A prey plasmid (pAD-LeFPP) was identified that led to activation of the two reporter genes HIS and LacZ after retransformation with the pBD-LeMAF1 bait.

  • 1Ashows that pAD-LeFPP leads to activation of the HIS gene in combination with pBD-LeMAF1 (sector a) and with pBD-AtMAF1 (sector b), but not with pBDAtRanGAP1 (sector c) or the binding domain vector pBD-GAL4 alone (sector d).

  • Although yeast two-hybrid interactions can not be easily quantified, the very high level of β-Galactosidase expressed by the pBD-LeMAF1/pAD-LeFPP stain suggests a comparably strong interaction in the yeast two-hybrid screen.


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