Example sentences for: pbad

How can you use “pbad” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • pJM127 was digested with Eco RI and Xba I to release a pcrG fragment that was subsequently subcloned into EcoRI- and XbaI-cleaved pBAD18-Kan to create pJM132.

  • Plasmid pJM127 was constructed by cloning an Eco RI-cleaved PCR p6roduct of pcrG into Eco RI- and Sma I-cleaved pBAD18.

  • Mutazyme (Stratagene, La Jolla, CA) was used to amplify PcrG for cloning into pBAD18 [ 33 ] . The ligation reaction was electroporated into lcrG Y. pestis (which is temperature sensitive due to its Ca 2+-blind phenotype) and the cells were plated on TMH medium [ 29 ] containing arabinose, Ca 2+, kanamycin and carbenicillin.

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