Example sentences for: orthovanadate

How can you use “orthovanadate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A549 cells or NHBEs were treated with CSC as described above and whole-cell protein extracts were prepared by scraping cells into lysis buffer containing 20 mM Tris-HCl (pH 8.0), 1 mM EDTA, 1 mM sodium orthovanadate, 1 mM sodium fluoride, 1 μg/ml each of leupeptin and pepstatin, and 1 μM PMSF.

  • At this time, okadaic acid (1 μM), sodium orthovanadate (1 μM) and PD098059 (10 μM) or vehicle (0.

  • 2 , 5 mM MnCl 2 , 100 μM sodium orthovanadate).

  • Frozen lung tissue was homogenized in ice cold 750 μl radioimmunoprecipitation assay (RIPA) buffer (1×PBS, 1% Igepal CA-630, 0.5% sodium deoxycholate, 0.1% SDS) plus protease inhibitors (30 μl/ml RIPA aprotinin, 10 μl/ml RIPA 100 mmol/l sodium orthovanadate).

  • Five hundred microliters of supplemented RIPA lysis buffer (50 mM Tris-HCI pH 7.5, 150 mM NaCI, 2 mM EGTA, 0.1% Triton X-100, 1 mM PMSF, 10 ug/ml aprotinin, 10 ug/ml leupeptin, 1 mM sodium orthovanadate) were added to each dish and cells were harvested with a rubber policeman.


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