Words similar to datp

Example sentences for: datp

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Two micrograms of total RNA was reverse transcribed with 32P-dATP, and the labeled fractions collected using ChromaSpin (human) columns.
Probes were 3'-end labeled with 35S or 33P-dATP using a terminal transferase reaction and hybridization carried out [ 26 ] . Autoradiograms were generated by dipping slides in NTB2 nuclear track emulsion and storing in the dark at 4°C.
The PCR product was digested with either the Eco RI or Xba I restriction enzyme, end-labeled with [αP 32]-dATP (ICN, Inc.) using the Klenow fragment of DNA polymerase I, and purified using a PCR clean-up kit (Qiagen).
The forward and reverse second round PCR primers were KK*ACAA*F2: 5'-AGTCAGGAATGGCTGCACC-3'and KK*ACAA*R: 5'-CAAATCAATC TTTGGGCAGG-3', respectively; the KK*ACAA*F2 primer was end-radiolabeled with 33P-dATP prior to PCR.
The oligo probes were radiolabeled using the Klenow fragment of DNA polymerase and 32P-dATP.