Words similar to μf
Example sentences for: μf
How can you use “μf” in a sentence? Here are some example sentences to help you improve your vocabulary:
Jurkat Tag cell lines (10 7cells) were transfected by electroporation using a Biorad electroporator at 250 V and 960 μF or by square wave electroporation on a BTX 830 using settings of 300 V and 10 ms.
All ES cell lines were cultured on mitotically inactivated primary embryonic fibroblasts in ES cell medium supplemented with recombinant leukemia inhibitory factor (LIF) as described elsewhere [ 22 ] . F3 cells were generated by introducing 100 μg of Not1 digested pΔHPRT plasmid into 1.5 × 10 8R1 ES cells by electroporation at 250 volts/500μf/resistance 8 using a BTX ECM600 electroporation system [ 7 ] . Cells containing neo were selected by supplementing the ES cell medium with 250 μg/ml Geneticin (G418, Gibco-BRL) and negative selection against Hsv-tk gene expression was achieved by including 2 μM gancyclovir (Roche).
This was introduced into 2.5 × 10 8ES cells by electroporation at 250 volts/500μf/resistance 8 using a BTX ECM600 electroporation system.
100 μg of pHDMCCre8 was introduced into 5 × 10 7ES cells by electroporation using 400 v/500μf/resistance setting 8. 1/100,000 of the total electroporated cell population was plated per 10 cm 2tissue culture dish in ES cell medium supplemented with LIF and grown until individual colonies could be collected.
Cell samples were placed into pre-chilled (4°C) 4 mm or 1 mm electroporation cuvettes (BTX Inc., San Diego, CA) and electroporated under the following conditions: 500 V/capacitance and resistance, 1250 μF capacitance timing, 720 ohms resistance, and 270 V for 4 mm gap cuvettes or 68 V for 1 mm gap cuvettes (field strength of ~700 Vcm -1).
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