Example sentences for: λgt

How can you use “λgt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 2 is a promoter-trap plasmid containing a promoterless lacZ gene isolated from λgt11 in which the Eco RI site in the 3' end of the lacZ gene was removed by site-directed polymerase-chain-reaction (PCR) mutagenesis (personal communication R. Van Landingham).

  • The 4.3-kb Hin d III- Xho I human genomic fragment described above was used as a probe to screen a λgt11 human liver cDNA library for the NPM3 cDNA.

  • Following isolation of pure plaques, the insert DNAs were prepared by PCR methods with the following conditions: 100 μl reactions containing 1X Pfu buffer (20 mM Tris-Cl, pH 8.75, 10 mM KCl, 2 mM MgSO 4 , 10 mM (NH 4 ) 2 SO 4 , 0.1% v/v Triton X-100, 100 μg/ml bovine serum albumin), 1 μM λgt11 forward and reverse primers (Promega, Madison, WI), 0.2 mM deoxyribonucleotides, and 5 Units of recombinant Pfu DNA Polymerase (Stratagene, La Jolla, CA).


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