Example sentences for: β-galactosidase

How can you use “β-galactosidase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Each construct from the cDNA library that tested positive for both lacZ and his reporter gene expression was also tested for the ability to activate directly β-galactosidase/ his reporter gene transcription without the presence of the bait construct, pGBT9/proHB-EGFEx.

  • Transient transfection of MDA-MB-231 breast cancer cells has been described previously [ 19 ] . Briefly, 2 μg 3TP-Luc together with 0.3 μg β-galactosidase expression plasmid were transfected into cells grown to 70-80% confluency in 6-well plates using LipofectAMINE reagent (Invitrogen) following the manufacturers protocol.

  • Colony filter-lift assays to detect β-galactosidase activity were performed as described by Clontech laboratories.

  • 5 embryos were generated from three independent αMHC-LacZ cell lines and stained for expression of β-galactosidase.

  • The plasmid pAdapt Luc, pAdpat LacZ, pAdapt hEndo and pAdapt mhAB encoding respectively for the luciferase, β-galactosidase, human endostatine and murinised human ATF-BPTI genes were constructed as described previously [ 23 ] . The different genes are under the control of the cytomegalovirus (CMV) immediate-early promotor and terminated by the simian virus (SV40) late poly(A) signal.


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