Words similar to αmhc

Example sentences for: αmhc

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Representative embryos, shown in fig 3dall developed normally and expressed high levels of β-galactosidase in the developing heart, mimicking the expression of endogenous αMHC [ 16 ] . Importantly, none of the embryos exhibited ectopic patterns of expression lending further support to the proposal that expression of transgenes introduced to the hprt locus is primarily dependent on the accompanying transcriptional regulatory elements [ 2 ] .
The 9.7 kb αMHCLacZ expression cassette was constructed by inserting a 3.6 kb SalI/HindIII fragment from pLacF containing a lacZ gene that incorporates a nuclear localization signal into the SalI/HindIII site of an αMHC expression module.
We chose the αMHC promoter because others have demonstrated, using traditional transgenic procedures, that it directs high levels of reporter gene expression specifically in cardiac tissue of early mouse embryos [ 16 17 18 ] . Cells were selected for resistance to HAT following electroporation of the α MHC-LacZ transgene-containing plasmid.
To assess the efficiency of this approach we attempted to target a LacZ reporter transgene whose expression is controlled by the promoter from the alpha myosin heavy chain (αMHC) gene to the modified hprt locus in F3 cells.