Example sentences for: αloxpneo

How can you use “αloxpneo” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Southern blot analysis (fig 3) shows that deletion of the pgk promoter in Hnf3αloxPNeo cells generated a predicted 3.5 kb HindIII/EcoRV fragment that was detected by probe A. Moreover, Fig 3cshows that deletion of the pgk promoter resulted in the Hnf3αloxPNeo cells reverting to G418-sensitivity because the promoter is necessary for Neo expression.

  • Next, a plasmid that expresses Cre-recombinase in mammalian cells was introduced into Hnf3αloxPNeo ES cells by transient transfection (materials and methods).

  • The pgk promoter was deleted from 25% of transfected Hnf3αloxPNeo ES cells, using the approach described in materials and methods.

  • These correctly targeted ES cell clones were called Hnf3αloxPNeo clones 1-4 to indicate that a pgkloxP-Neo cassette had been introduced into the Hnf3 α locus.


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