Example sentences for: zvad
How can you use “zvad” in a sentence? Here are some example sentences to help you improve your vocabulary:
The essential step in plasma membrane destabilization and MP-release very likely is a caspase-dependent sphingomyelin hydrolysis, which leads to the production of ceramide [ 28 29 30 ] . It is possible, however, that sphingomyelin hydrolysis and MP-release may be a consequence of the action of initiator caspases or other ZVAD sensitive proteases rather than only executioner caspases [ 15 31 32 ] . Our study was performed in HUVEC culture, which may have particular properties in respect of MP release when compared with endothelial cells from other organs.
Similarly, induction of apoptosis with 5 μM CPT, a topoisomerase I inhibitor [ 19 ] , led to a release of annexin V-binding MP which was prevented by ZVAD.
AGTII, angiotensin II; BSA, bovine serum albumin; CHX, cycloheximide; CPT, camptothecin; DMSO, dimethyl sulfoxide; FITC, fluorescein isothiocyanate; HBSS, Hanks' balanced salt solution; HUVEC, human umbilical vein endothelial cells; IC-IgG, IgG isotype control; Mab, monoclonal antibody; MP, microparticles; PE, phycoerythrin; PI, propidium iodide; PMA, phorbol-myristate-acetate; PS, phosphatidylserine; TRAP, thrombin receptor activating peptide (SFLLRNP); TNF, tumor necrosis factor; TPA, tissue plasminogen activator; TUNEL, terminal deoxynucleotidyltransferase dUTP nick end labeling; ZVAD, Z-Val-Ala-Asp-fluoromethyl ketone.
ZVAD also inhibited a release of MP from unstimulated cells, which probably represents spontaneous apoptosis of cultured HUVEC (Fig.
Louis, MO) 5 μmol/L or 500 nmol/L; CPT with caspase inhibitor Z-Val-Ala-Asp-fluoromethyl ketone (ZVAD) from Calbiochem (San Diego, CA) 50 μmol/L or ZVAD 50 μmol/L alone.
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