Example sentences for: zona-free

How can you use “zona-free” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Indirect immunofluorescence with zona-free Eggs

  • When zona-free mature eggs were incubated with anti-GRP94, the antibody specifically bound to isolated regions of the plasma membrane in several eggs; however, the majority of eggs did not show any reactivity (Fig.

  • For these experiments, proteins from 800 zona-free biotinylated mouse eggs were extracted.

  • Another 800, untreated zona-free eggs were extracted, separated by 2D electrophoresis and the gel was silver-stained.

  • This procedure is explained in detail in Coonrod et al, 1999 [ 8 ] . Briefly, after collection zona-free eggs were washed six times in BWW medium (Irvine Scientific, Santa Ana, CA) containing 100 μg/ml polyvinylalcohol (PVA, Sigma, St. Louis, MO), biotinylated with 2 mg/ml Sulfo-NHS biotin (Pierce, Rockford, IL) in BWW/PVA for 7 min at room temperature, and washed six times in BWW/PVA.


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