Example sentences for: zd

How can you use “zd” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Therefore the ZD domain can supply the function that is missing in Top67 even when the two domains are not covalently linked.

  • Top67 and the ZD domains at different concentrations were mixed and incubated at 37°C for 10 min before addition to the 0.3 μg of supercoiled plasmid DNA in 20 μl of 10 mM Tris-HCl pH 8.0, 2 mM MgCl 2 , 0.1 mg/ml gelatin.

  • E. coli DNA topoisomerase I and the ZD domain were expressed and purified as described [ 6 41 ] . To express the 67 kDa N-terminal transesterification domain (Top67), a stop codon at amino acid 598 was introduced into plasmid pJW312 [ 42 ] used for topoisomerase I expression by site-directed mutagenesis employing the Chameleon-Mutagenesis kit from Stratagene.

  • Our results showed that adding the purified ZD domain partially restored the relaxation activity.

  • This reaction involves passage of another double-stranded DNA circle, instead of the complementary DNA strand through the break generated by DNA cleavage so the ZD domain would not be expected to play any significant role.


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