Words similar to zd

Example sentences for: zd

How can you use “zd” in a sentence? Here are some example sentences to help you improve your vocabulary:

E. coli topoisomerase I can catalyze catenation of double-stranded DNA circles if the molecules contain single-strand scissions [ 15 16 ] . To test if the Top67 can carry out double-stranded DNA passage at enzyme cleavage sites across from the DNA nicks, the yield of DNA catenanes were compared with that obtained with topoisomerase I. In contrast to the relaxation activity, the catenating activity of Top67 shown in figure 6was as efficient as that of full-length topoisomerase I, and the addition of the ZD domain had no effect (Figure 6a).
Addition of the ZD domain had no effect on the cleavage selectivity of Top67.
We propose that the ZD domain interacts with the passing single-strand of DNA in the relaxation of negatively supercoiled DNA by this enzyme.
Although it is known that the ZD domain can function as a DNA binding domain, its exact role in these individual steps of removal of a negative superhelical turn from DNA by E. coli topoisomerase I remains to be defined.
Top67 and the ZD domains at different concentrations were mixed and incubated at 37°C for 10 min before addition to the 0.3 μg of supercoiled plasmid DNA in 20 μl of 10 mM Tris-HCl pH 8.0, 2 mM MgCl 2 , 0.1 mg/ml gelatin.