Words similar to ypd

Example sentences for: ypd

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The same crosses were also used to determine if Tab +was recessive or dominant: for every tab mutant, haploid cdc15Δ tab [pMET3-cdcl5-2, URA3] and diploid cdc15Δ/cdc15Δ tab/+ [pMET3-cdc15-2, URA3 ] cells were pre-grown on YPD (1% yeast extract/2% peptone + 2% glucose) at 25°C for 2-3 days, spotted in 5-fold serial dilutions onto SD+5-FOA plates, and the number of 5-FOA resistant colonies from the diploid was divided by that from the haploid to obtain ratio P. The mutant was defined to be dominant if P= 0.1-1, semi-dominant if P= 0.01-0.
While NetSearch correctly identified the upstream elements of this pathway (Sln1p → Ypd1p → Ssk1p → Ssk22p), it was unable to form any connections to Pbs2p or Hog1p that ended in a transcription factor.
Diploids were then replica plated back to YPD and assayed for growth at 34°.
All yeast strains used in this study are listed in Table 1. General yeast manipulations were conducted by standard methods [ 62 ] with transformations by the lithium acetate method [ 63 ] . All strains were grown at 23° in YPD (yeast extract, peptone, 2% glucose) unless otherwise noted.
For the experiment described in Figure 1, cdc15-2 (RJD 619) cells were grown to exponential phase in YPD at 25°C, and shifted to 37°C for three hours so that >95% cells were arrested as large-budded cells.