Words similar to ypd

Example sentences for: ypd

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Mutants were grown at 23° overnight in 5 ml YPD to logarithmic phase.
To test temperature sensitivity (ts) of tab mutants, cells were spotted or streaked onto YPD plates, and incubated at 37°C.
Cells were grown in 1% yeast extract/ 2% peptone (YP) with 2% glucose (YPD), 2% galactose (YPG), or 2% raffinose (YPR).
The same crosses were also used to determine if Tab +was recessive or dominant: for every tab mutant, haploid cdc15Δ tab [pMET3-cdcl5-2, URA3] and diploid cdc15Δ/cdc15Δ tab/+ [pMET3-cdc15-2, URA3 ] cells were pre-grown on YPD (1% yeast extract/2% peptone + 2% glucose) at 25°C for 2-3 days, spotted in 5-fold serial dilutions onto SD+5-FOA plates, and the number of 5-FOA resistant colonies from the diploid was divided by that from the haploid to obtain ratio P. The mutant was defined to be dominant if P= 0.1-1, semi-dominant if P= 0.01-0.
A total of 54 single colonies derived from the 16 heterozygotes were inoculated into separate YPD liquid cultures.