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Example sentences for: yopd

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In addition, YopN, TyeA, SycN, YscB, LcrH, YopD, and LcrQ are all also required for the negative regulation of Yops secretion [ 6 7 8 9 10 11 12 13 14 15 16 17 ] . LcrV (the V antigen) is thought to positively regulate Yops secretion as lcrV strains do not secrete Yops, even under conditions that normally favor secretion [ 18 19 ] .
Proteins resolved by SDS-PAGE were transferred to Immobilon-P membranes (Millipore Corp., Bedford, MA) using carbonate transfer buffer (pH 9.9) [ 5 ] . Specific proteins were visualized using rabbit polyclonal antibodies specific for glutathione S-transferase (GST)-tagged LcrG (α-LcrG) [ 4 ] , His-tagged LcrV (α-LcrV) [ 4 ] , YopM (α-YopM) [ 36 ] , GST-tagged LcrQ (α-LcrQ) [ 16 ] , YopN (aka LcrE) (α-LcrE [ 4 ] ), His-tagged YopD (α-YopD) [ 16 ] , and YopE (α-YopE; gift from S. C. Straley, University of Kentucky, Lexington, KY.