Words similar to yopd

Example sentences for: yopd

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Proteins resolved by SDS-PAGE were transferred to Immobilon-P membranes (Millipore Corp., Bedford, MA) using carbonate transfer buffer (pH 9.9) [ 5 ] . Specific proteins were visualized using rabbit polyclonal antibodies specific for glutathione S-transferase (GST)-tagged LcrG (α-LcrG) [ 4 ] , His-tagged LcrV (α-LcrV) [ 4 ] , YopM (α-YopM) [ 36 ] , GST-tagged LcrQ (α-LcrQ) [ 16 ] , YopN (aka LcrE) (α-LcrE [ 4 ] ), His-tagged YopD (α-YopD) [ 16 ] , and YopE (α-YopE; gift from S. C. Straley, University of Kentucky, Lexington, KY.
In addition, YopN, TyeA, SycN, YscB, LcrH, YopD, and LcrQ are all also required for the negative regulation of Yops secretion [ 6 7 8 9 10 11 12 13 14 15 16 17 ] . LcrV (the V antigen) is thought to positively regulate Yops secretion as lcrV strains do not secrete Yops, even under conditions that normally favor secretion [ 18 19 ] .