Example sentences for: yeast

How can you use “yeast” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In addition, BRCA1 can act as a transcriptional transactivator in yeast reporter assays [ 26, 27] and binds the RNA polymerase II holoenzyme, a component of the basal transcription machinery [ 25].

  • When cells harboring conditional-lethal temperature-sensitive (ts) mutations in any of these proteins are shifted to the restrictive temperature, they uniformly arrest in late anaphase as large-budded cells with segregated chromosomes, fully elongated microtubule spindles, and in all tested cases, elevated Cdc28/Clb2 protein kinase activity (Cdc28 is the major cyclin-dependent kinase Cdk1 and Clb2 the major mitosis-specific cyclin of budding yeast) (reviewed in [ 1 ] , [ 2 ] ).

  • pVT725 was created by replacing the ampicillin resistance cassette on pLexA (Clontech) with a kanamycin resistance gene (obtained from pGBKT7 (Clontech) by PCR) via homologous recombination in yeast.

  • Negative genetic selection eliminates the background due to vector recircularization caused by end-joining during yeast transformation.

  • RGS3T is comprised primarily by the consensus RGS domain and was originally reported to decrease the growth rate of yeast and to inhibit IL-8 receptor mediated activation of MAP kinase by a factor of 3-10 fold as compared to RGS3 [ 24 ] and was subsequently reported to be a normally expressed variant of RGS3 [ 37 ] . Therefore, we compared it to RGS3 for attenuation of GnRH-stimulated IP 3 production in COS-1 cells (Fig.


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